Antimicrobial efficacy of S-PRG containing toothpastes on S. mutans biofilm development

Objective: to investigate the antimicrobial effects of toothpastes containing bioactive surface pre-reacted glass particles (S-PRG) on S. mutans biofilms adherence, initial colonization and maturation. Material Methods: a reference UA 159 clinical (SM6) strain were used. Bovine enamel specimens randomly allocated into groups (n=5): 0%; 1%; 5%; 20%; 30% S-PRG; positive control dentifrice (NaF+triclosan); negative (distilled water). For biofilm development, samples placed in 24-well plate artificial saliva (4h), followed by adding 1mL saliva, BHI broth 225uL suspension. Treatments with applied previously or after 4h 24h formation. Samples incubated for 48h at 37°C 5%CO2 was detached seeded Petri dishes determining number viable cells. Data analyzed ANOVA Tukey test (5%). Results: significantly lower microorganisms’ adherence (p<0.05) obtained all S-PRG toothpastes, similar results NaF+triclosan SM6 20 30%S-PRG exhibiting higher inhibition effect than NaF+Triclosan UA159. Antibacterial early-stage also observed groups, but not superior toothpaste. mature biofilm, effective potential only strain, control. Conclusion: experimental inhibit growth activity, being promising agents prevent cariogenic development. KEYWORDS Biofilm; Dental enamel; Giomer; Streptococcus mutans, S-PRG.